Fig. 3
From: Exposure of A2E to blue light promotes ferroptosis in the retinal pigment epithelium
Iron-chelating agent DFP relieves blue light-induced ferroptosis in A2E-loaded ARPE-19 cells. A Cell viability (n = 6). ARPE-19 cells that were incubated with 25 μM A2E for 48 h were pretreated with serial concentrations of DFP (50, 100, and 200 μM) for 2 h and then illuminated by blue light for 30 min, followed by 24 h of incubation. B−E ARPE-19 cells that were treated for 48 h with 25 μM A2E were preincubated with 100 μM DFP for 2 h and exposed for 30 min to blue light, followed by 24 h of incubation. B LDH release detection (n = 6). C FeRhoNox-1 staining (n = 3). D H2DCFDA staining (n = 3). E Western blots of SLC7A11 and GPX4 (n = 3). Scale bars in C and D, 20 μm