Fig. 2

GSH depletion mediates blue light-induced ferroptosis in A2E-loaded ARPE-19 cells. A GSH detection (n = 6). ARPE-19 cells were incubated with 25 μM A2E for 48 h and then exposed to blue light for 30 min, followed by 24 h of incubation. B Cell viability (n = 6). ARPE-19 cells that were incubated with 25 μM A2E for 48 h were pretreated with serial concentrations of GSH (2, 4, and 6 mM) for 1 h and then illuminated by blue light for 30 min, followed by 24 h of incubation. C − E ARPE-19 cells that were treated for 48 h with 25 μM A2E were preincubated with 4 mM GSH for 1 h and exposed for 30 min to blue light, followed by 24 h of incubation. C H2DCFDA staining (n = 3). Scale bars, 20 μm. D Lipid peroxidation (n = 3). Scale bars, 10 μm. E Western blots of SLC7A11 and GPX4 (n = 3)