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Fig. 5 | Cellular & Molecular Biology Letters

Fig. 5

From: SRSF3 and hnRNP A1-mediated m6A-modified circCDK14 regulates intramuscular fat deposition by acting as miR-4492-z sponge

Fig. 5

SRSF3 promotes nuclear export of m6A-modified circCDK14. A Prediction of RBPs and potential m6A sites of circCDK14; B MeRIP showing m6A antibody enrichment for circCDK14; C and D the SRSF3 mRNA (C) and protein (D) expression level after SRSF3 interference; E SRSF3 antibody for circCDK14 enrichment; after SRSF3 interference, F circCDK14 enrichment by SRSF3 antibody; G circCDK14 enrichment by m6A antibody; H circCDK14 expression in the nucleus and cytoplasm; I CCK-8 assay; J EdU staining; K expression levels of differentiation marker genes; L expression level of proliferation marker genes; M BODIPY staining; N Oil Red O staining. The western blot, RIP and MeRIP were repeated 3 times (n = 3), CCK-8 assay was repeated 6 times (n = 6) and other experiments were performed in triplicate and repeated 3 times (n = 9)

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