Fig. 3

CircCDK14 regulates yak IMF deposition through miR-4492-z. A Dual-luciferase assay to detect miR-4492-z binding to circCDK14; co-transfection with circCDK14 siRNA versus a control siRNA and either the miR-4492-z inhibitor or a control, B CCK-8 assay; C EdU staining; D expression level of proliferation marker genes; E expression levels of differentiation marker genes; F Oil Red O staining; G BODIPY staining; H–I expression of FBXO17 after interference or overexpression with circCDK14; J dual-luciferase assay for detection of miR-4492-z binding to FBXO17; K–L expression of FBXO17 after overexpression or interference of miR-4492-z. The CCK-8 assay was repeated 6 times (n = 6), and the other experiments were performed in triplicate and repeated 3 times (n = 9)