Fig. 4

MLN4924 stabilizes CRBN by increasing KDM5C. A HEK293T cells were transfected with the FLAG-KDM5C plasmid for 24 hand treated with MLN4924 (1 µM) for 24 h, and whole-cell lysates were immunoblotted for KDM5C. B MLN4924 did not increase CRBN in KDM5C deficient HEK293T cells. HEK293Tcells were transfected with siKDM5Cfor 48 h, treated with MLN4924 (1 µM) for 24 h, and lysed for Western blotting. C HEK293T cells were treated with 1 µM MLN4924 for 12 h, and then the cells were treated with the 100 µg/mL CHX for the indicated time. The data are shown as the means ± SEMs (n = 3), Student’s t test,*P < 0.05;****P < 0.0001