Fig. 3

The small-molecule compound MLN4924 increases KDM5C and CRBN. A HEK293T cellswere treated with various types of inhibitors (1 µM MLN4924, 1 µM Btz, 1 µM MG132, 100 nM Baf A1, 1 µM Y-27632, 1 µM AA, and 1 µM 2-BP) for 24 h and the resulting cell lysates were subjected to western blotting. B–E MLN4924 (1 µM) was used to treat HeLa, HT22 (B), THP-1 (C), NB4 (D), and K562 (E) cells for 24 h and the cell lysates were used for western blotting. The data are shown as the means ± SEMs (n = 3).Student’s ttest, *P < 0.05; **P < 0.01; ns: not significant