Fig. 1

The histone demethylase KDM5C interacts with CRBN. A The CRBN-interacting proteins were labeled with biotin, purified with NeutrAvidin, digested with trypsin, and analyzed by LC‒MS/MS. B Information for MS-identified tryptic peptides from KDM5C. C HA-CRBN interacted with FLAG-KDM5C. HEK293T cells were transfected with the indicated plasmids for 48 h, collected, lysed, and subjected to immunoprecipitation assay and western blotting. D Exogenous CRBN interacted with endogenous KDM5C. The plasmids of pcDNA3.1 or FLAG-CRBN were transfected into HEK293T cells for 48 h. The cells were collected, lysed, and subjected to immunoprecipitation and western blotting. E, F Endogenous KDM5C interacted with endogenous CRBN in AML cells. NB4 (E) or THP-1 (F) cells were collected, lysed, and subjected to immunoprecipitation and western blotting using the indicated antibodies