Fig. 6
Knockdown of CDH17 promotes the proliferation and cisplatin sensitivity of CTC-TJH-01 cell clusters. A Morphological observation of CTC-TJH-01 cells after knockdown of the CDH17 gene. B Detection of YAP signaling pathway related proteins after CDH17 knockdown via western blotting. C The proliferative activity of CTC-TJH-01 cells after CDH17 knockdown was detected via a CCK-8 assay. D When the CDH17 gene was knocked down in suspension-cultured CTC-TJH-01 cells treated with cisplatin, apoptosis was detected by flow cytometry. E When the CDH17 gene was knocked down in suspension-cultured CTC-TJH-01 cells treated with cisplatin, the expression of caspase 3 and survivin was analyzed by western blotting. F A representative image of a tumor excised 57 days after subcutaneous transplantation of a suspension of cultured CTC-TJH-01 cells (1 × 106 cells) into NOD-SCID mice (n = 6). G Tumor volume was measured with a digital caliper. H Tumor weight was measured with an electronic balance. I HE staining of CTC-TJH-01 cell cluster xenograft tumor tissues. Scale bar, 120 μm. J Immunohistochemical analyses of CDH17 and Ki-67 expression levels in CTC-TJH-01 cell cluster xenograft tumor tissues. Scale bar, 120 μm. Each bar represents the mean ± SD of three separate experiments. *P < 0.05; **P < 0.01; ***P < 0.001