Fig. 4
Comparison of the sensitivity of CTC-TJH-01 cells to cisplatin in adherent and suspension cultures in vivo. A Adherent and suspension-cultured CTC-TJH-01 cells (1 × 106 cells) were subcutaneously transplanted into nude mice, which were subsequently treated with cisplatin (2 mg/kg) for 5 continuous days, for a total of two treatments. The tumors were excised and photographed on the 67th day (n = 6). B Tumor weight was measured with an electronic balance. C Tumor growth was measured with a digital caliper and tumor growth curves were drawn. D Mouse body weights were measured twice a week. E HE staining of CTC-TJH-01 cell xenografts. Scale bar, 100 μm. F Immunohistochemical analyses of Ki-67 expression levels in CTC-TJH-01 cell xenograft tumor tissues. Scale bar, 100 μm. Each bar represents the mean ± SEM of three separate experiments. *P < 0.05; **P < 0.01; ***P < 0.001