Fig. 2
Comparison of the tumorigenicity of CTC-TJH-01 cells in adherent and suspension cultures in vivo. A A representative image of tumors excised 62 days after subcutaneous transplantation of adherent and suspension-cultured CTC-TJH-01 cells (5 × 105 cells) into nude mice (n = 8). B Tumor-seeding ability of CTC-TJH-01 cells after suspension and adherent culture. C Tumor weight was measured with an electronic balance. D Growth curves of the tumors arising from the inoculation of adherent and suspension cultured CTC-TJH-01 cells. Tumor growth was measured with a digital caliper. E Tumorigenesis time of adherent and suspension cultured CTC-TJH-01 cells. F HE staining of adherent and suspension-cultured CTC-TJH-01 cell xenografts. Scale bar, 100 μm. G Immunohistochemical analyses of Ki-67 expression levels in adherent or suspension-cultured CTC-TJH-01 tumor tissues. Scale bar, 100 μm. Each bar represents the mean ± SEM of three separate experiments. *P < 0.05; **P < 0.01; ***P < 0.001