Fig. 1
Comparison of the biological characteristics of CTC-TJH-01 cells in different growth states. A Morphological observation of the CTC-TJH-01 cells after adherence and suspension culture. Scale bar, 200 μm. B Comparison of the proliferation ability of adherent and suspension cultured CTC-TJH-01 cells. C Analysis of the cell cycle distribution of CTC-TJH-01 cells after adherent and suspension culture. D Flow cytometry analysis of the proportion of quiescent cells in adherent and suspension cultures of CTC-TJH-01 cells. E Comparison of the proliferative activity of adherent-cultured CTC-TJH-01 cells cultured with the supernatants of adherent-cultured and suspension-cultured CTC-TJH-01 cells. F Analysis of differential gene heatmaps related to the cell cycle and DNA replication signaling pathways in CTC-TJH-01 cells cultured in adherent and suspension cultures after transcriptome sequencing. G Expression of cell proliferation related proteins in adherent and suspension cultured CTC-TJH-01 cells. H Expression of cancer stem cell related proteins in adherent and suspension-cultured CTC-TJH-01 cells. I The expression of CD44 and SOX2 proteins in adherent and suspension-cultured CTC-TJH-01 cells by immunofluorescence staining. Scale bar, 25 μm. Each bar represents the mean ± SD of three separate experiments. *P < 0.05; **P < 0.01; ***P < 0.001