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Fig. 6 | Cellular & Molecular Biology Letters

Fig. 6

From: Heterogeneous focal adhesion cytoskeleton nanoarchitectures from microengineered interfacial curvature to oversee nuclear remodeling and mechanotransduction of mesenchymal stem cells

Fig. 6

Heterogeneous focal adhesion (FA) assembly and cytoskeleton remodeling to regulate yes-associated protein (YAP) mechanotransduction and nuclear laminA/C organization in an engineered colony of hMSCs. a Fluorescent pictures of YAP staining (green), nuclei were stained blue, scale bar represents 1000 μm, bottom two panels are enlargement images of central and peripheral regions, scale bar represents 500 μm; b percentage of YAP nuclear location at the central regions; c percentage of YAP nuclear location at the peripheral regions, data are presented as mean ± standard deviation (SD), n = 5, n.s.: no significance, *p < 0.05, **p < 0.01, ***p < 0.001; d Western blot (WB) analysis of laminA/C in the engineered colony with low and high density; e quantitative laminA/C/β-actin in the central regions; f quantitative laminA/C/β-actin in peripheral regions, data are presented as mean ± SD, n = 3, n.s.: no significance, ***p < 0.001, ****p < 0.0005

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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