Fig. 6

MiR-26b-5p inhibits BCa cell growth in vivo by targeting KPNA2. A, B On the basis of the median level of KPNA2, a distribution map of DEGs was generated, identifying a total of 1032 DEGs, with 544 upregulated and 488 downregulated. Gene Set Enrichment Analysis (GSEA) indicated that the DEGs are closely linked to the PI3K/AKT signaling pathway. C Western blotting to detect the expression of key genes of the PI3K/AKT pathway. D Nude Mouse Subcutaneous Tumor Model Diagram. E Measurement of tumor volume at specified time points and upon completion of the experiment, mice were euthanized, and the weight of subcutaneous tumors was recorded. F Immunohistochemical staining of Ki-67, KIFC1 and KPNA2 in tumors implanted in mice. *, p < 0.05; **, p < 0.01; ***, p < 0.001