Fig. 4

MiR-26b-5p induces G2/M phase arrest and promotes BCa cell apoptosis. A Using flow cytometry to investigate the impact of miR-26b-5p and KPNA2 on the cell cycle of BCa cells. The study results indicated that overexpression of miR-26b-5p in J82 and T24 cells significantly reduced the number of cells in the G2/M phase, while supplementation of KPNA2 partially reversed this effect. Conversely, inhibiting miR-26b-5p in 5637 cells led to a significant increase in cells in the G2/M phase, which could be partially counteracted by targeted knockout of KPNA2. B In apoptosis assays, higher levels of miR-26b-5p led to a notable rise in the proportion of apoptotic cells in J82 and T24 cells. Introducing exogenous KPNA2 partially reverses this impact, leading to a reduction in the proportion of apoptotic cells. Lowering miR-26b-5p expression reduces the proportion of apoptotic cells in 5637 cells, whereas the depletion of KPNA2 partially reinstates the proportion of apoptotic cells. *, p < 0.05; **, p < 0.01; ***, p < 0.001