Fig. 2

MiR-26b-5p inhibits the growth and proliferation ability of BCa cells. A KPNA2 levels were analyzed using RT-qPCR in five BCa cell lines (5637, UMUC-3, J82, T24, and SW780) and an immortalized normal uroepithelial cell line (SV-HUC-1). B Western blot analysis of KPNA2 protein abundance in the same five BCa cell lines and the immortalized normal urinary tract epithelial cell line (SV-HUC-1). C Validation of the transfection efficiency of miR-26b-5p mimic in J82 and T24 cell lines and miR-26b-5p inhibitor in 5637 cell lines using RT-qPCR. D KPNA2 protein abundance was verified by western blot in J82 and T24 cell lines with miR-26b-5p-specific mimics transfected and 5637 cell lines with miR-26b-5p-specific inhibitor transfected. E, F CCK-8 assays and colony formation assays showed that miR-26b-5p mimic inhibited the growth and proliferation ability of J82 and T24 cells, and miR-26b-5p inhibitor promoted the growth and proliferation ability of 5637 cells. *, p < 0.05; **, p < 0.01; ***, p < 0.001