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Fig. 5 | Cellular & Molecular Biology Letters

Fig. 5

From: Feedback loop centered on MAF1 reduces blood–brain barrier damage in sepsis-associated encephalopathy

Fig. 5

Overexpression of PTEN attenuated the RIG-I/IRF3 signal pathway and apoptosis and recovered normal autophagy. The rBMECs and rAstrocytes were treated with 10% serum harvested from the sham (sham-S) or model rats (model-S). A, B qPCR and western blot analyses of MAF1 and PTEN expression in the sham-S + vector, model-S + vector, and model-S + PTEN groups. C Western blot analyses of AKT/mTOR signaling pathway. D MAF1 and CUL2 expression was measured by IF. E Western blot analyses of proteins RIG-I and IRF3, caspase-3, p62, and LC3B. F, G qPCR and flow cytometry were used to detect IFNβ expression levels. H CCK-8 analysis of rBMEC and rAstrocyte activity at 0, 6, 12, 18, and 24 h after transfection. I Flow cytometry analysis of apoptosis in transfected cells. J Transmission electron microscopy was used to detect autophagy in rBMECs and rAstrocytes. ***P < 0.001 model-S + vector versus sham-S + vector. #P < 0.05, ###P < 0.001 model-S + PTEN versus sham-S + vector

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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