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Fig. 6 | Cellular & Molecular Biology Letters

Fig. 6

From: Exercise promotes skeletal muscle growth in adolescents via modulating Mettl3-mediated m6A methylation of MyoD in muscle satellite cells

Fig. 6

Increased expression of MyoD could contribute to myogenesis of adolescent MuSCs. a The relative mRNA expression of MyoD in adolescent MuSCs after transfected with pcDNA-MyoD-WT (MyoD) or empty plasmid (vector) (n = 3). b The protein levels of MyoD and GAPDH in adolescent MuSCs after transfection with pcDNA-MyoD-WT (MyoD) or empty plasmid (vector) (n = 4). c Immunofluorescence staining of MyHC (red) with differentiated adolescent MuSCs. Adolescent MuSCs were differentiated for 24 h after transfection with pcDNA-MyoD-WT (MyoD) or empty plasmid (vector). Nuclei were labeled with DAPI. Scale bars, 50 μm. d Statistical analysis of the average diameter of myotubes from differentiated adolescent MuSCs transfected with pcDNA-MyoD-WT (MyoD) or empty plasmid (vector) (n = 5). e Statistical analysis of the differentiation efficiency for adolescent MuSCs transfected with pcDNA-MyoD-WT (MyoD) or empty plasmid (vector) (n = 5). f The relative mRNA expression of Myh1 and Mck in differentiated adolescent MuSCs transfected with pcDNA-MyoD-WT (MyoD) or empty plasmid (vector) (n = 3). g Immunofluorescence staining of MyoD (red) and MyoG (green) in tibialis anterior (TA) muscle from control and exercise mice at 8 weeks old. Scale bar, 25 μm. h Statistical analysis of MyoD+/MyoG+ cells in tibialis anterior (TA) muscle from control and exercise mice at 8 weeks old (n = 6). i Heat map of expression of Mymx and Mymk between control and exercise mice. j The relative mRNA expression of Mymx and Mymk in tibialis anterior (TA) muscle from control and exercise mice at 8 weeks old (n = 3). Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ns indicates no significant changes

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