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Fig. 5 | Cellular & Molecular Biology Letters

Fig. 5

From: Salivary gland protective and antiinflammatory effects of genistein in Sjögren’s syndrome by inhibiting Xist/ACSL4-mediated ferroptosis following binding to estrogen receptor-alpha

Fig. 5

Genistein reduced inflammation and ferroptosis by upregulating XIST-mediated ACSL4 gene silencing in SGECs. A The level of RNA expression of XIST in SGECs. B The transfection efficiency of si-XIST in SGECs by the RT-qPCR assay. C, D The expression levels of ACSL4 proteins evaluated by western blotting. E Fluorescence in situ hybridization (FISH) for XIST (red) and ACSL4 (green) mRNA colocalization in SGECs. F Western blotting of AQP5, and SSB proteins in XIST knockdown SGECs. The MDA (G) and GSH (H) levels in XIST knockdown SGECs. I, J Representative images of FerroOrange staining in XIST knockdown SGECs. K The XIST expression pattern in genistein-treated XIST-knockdown SGECs. L Western blotting of ACSL4 proteins in genistein-treated XIST-knockdown SGECs. The MDA (M) and GSH levels (N) in genistein-treated XIST-knockdown SGECs. O, P Representative images of FerroOrange staining in genistein-treated XIST-knockdown SGECs. Significant differences are indicated as: *p < 0.05, **p < 0.01, and ***p < 0.001; n = 3

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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