Your privacy, your choice

We use essential cookies to make sure the site can function. We also use optional cookies for advertising, personalisation of content, usage analysis, and social media.

By accepting optional cookies, you consent to the processing of your personal data - including transfers to third parties. Some third parties are outside of the European Economic Area, with varying standards of data protection.

See our privacy policy for more information on the use of your personal data.

for further information and to change your choices.

Skip to main content
Fig. 4 | Cellular & Molecular Biology Letters

Fig. 4

From: SENP3 mediates deSUMOylation of SIX1 to promote prostate cancer proliferation and migration

Fig. 4

SENP3 interacts with SIX1 in PCa cells. A Schematic diagram of endogenous SIX1 and IgG immunoprecipitation combined with mass spectrometry on PC3. B Ambipolar ion peaks of SENP3 are shown. C Co-IP assay was performed using SIX1 antibody and control IgG antibody, which subjected to immunoblot for SENP3 and SIX1. D CO-IP assay was performed using SENP3 antibody and control IgG antibody, which subjected to immunoblot for SENP3 and SIX1. E Immunofluorescence assay was performed using FLAG-tag and HA-tag antibodies in the indicated 22Rv1 cells transfected with FLAG-SENP3 and HA-SIX1 plasmids (scale bar, 10μm). F Full-length (FL) and truncated mutants of SIX1 fused with HA-tag were engineered and co-transfected with FLAG-SENP3 into HEK293T cells for 48 h. G Co-IP assay was performed using FLAG-tag antibody beads and immunoblotted for FLAG, HA

Back to article page