Fig. 4
From: SENP3 mediates deSUMOylation of SIX1 to promote prostate cancer proliferation and migration

SENP3 interacts with SIX1 in PCa cells. A Schematic diagram of endogenous SIX1 and IgG immunoprecipitation combined with mass spectrometry on PC3. B Ambipolar ion peaks of SENP3 are shown. C Co-IP assay was performed using SIX1 antibody and control IgG antibody, which subjected to immunoblot for SENP3 and SIX1. D CO-IP assay was performed using SENP3 antibody and control IgG antibody, which subjected to immunoblot for SENP3 and SIX1. E Immunofluorescence assay was performed using FLAG-tag and HA-tag antibodies in the indicated 22Rv1 cells transfected with FLAG-SENP3 and HA-SIX1 plasmids (scale bar, 10μm). F Full-length (FL) and truncated mutants of SIX1 fused with HA-tag were engineered and co-transfected with FLAG-SENP3 into HEK293T cells for 48 h. G Co-IP assay was performed using FLAG-tag antibody beads and immunoblotted for FLAG, HA