Fig. 3
From: SENP3 mediates deSUMOylation of SIX1 to promote prostate cancer proliferation and migration

Inhibition of SENP3 impeded PCa migration. A–C The SENP3 mRNA levels were analyzed on the basis of three different GSE datasets. Unit: MAS5-calculated signal intensity (GSE3325), MAS5.0 signal intensity (GSE32269), and normalized log2 ratio (Cy5/Cy3) representing test/reference (GSE38241). PT, prostate tissue; PCa, prostate cancer. D and E Transwell migration assay was performed in 22Rv1 and PC3 cells transfected with SENP3 siRNAs or control for 24 h. Representations are shown (scale bars, 50 μm). F and G Scratch assay was performed in 22Rv1 and PC3 cells transfected with SENP3 siRNAs or control for 12 h or 72 h. Representations are shown (scale bars, 50 μm). H Western blot assay was performed to test expression of SENP3, N-cadherin, E-cadherin, GAPDH. *P < 0.05, **P < 0.01, ***P < 0.001, #P < 0.0001