Fig. 6

Atg5 deletion inhibits FGF2 production in renal tubules after I/R. The kidneys of WT and PT-atg5 KO mice were harvested at the indicated time points after sham surgery or bilateral I/R injury to detect the levels of the profibrotic factor FGF2. A Kidney slices were immunostained with antibody against FGF2 (red), DAPI to visualize the cell nuclei (blue), and LTL to visualize the proximal tubules (green). Scale bars, 20 μm. B Quantitative analysis of FGF2 positive areas. The dates are expressed as mean ± SD (n = 6). * represents a significant difference from the sham group; # represents a significant difference from the relevant wild-type group. # or *P < 0.05, ## or **P < 0.01, ### or ***P < 0.001, #### or ****P < 0.0001