Fig. 1
From: Dynamic regulation of proximal tubular autophagy from injury to repair after ischemic kidney damage
Atg5 deletion in proximal tubules exacerbates acute renal injury but reduces fibrosis during the recovery phase after I/R. WT and PT-atg5 KO mice were subjected to a sham operation or bilateral renal ischemia followed by reperfusion for up to 4 weeks (n = 5–10 mice per group), and the kidneys were collected at the indicated time points. A, B Representative immunoblots and quantification of ATG5, p62, and LC3 expression in the kidneys (n = 3). C Serum creatinine and BUN levels (n = 10). D Representative images of H&E staining, Masson’s trichrome and Sirius red staining. Scale bars, 50 μm. E, F Quantitative analysis of Masson’s trichrome and Sirius Red staining (n = 6). The values are expressed as mean ± standard deviation (SD), * represents a significant difference from the sham groups, and # represents a significant difference from the relevant wild-type group. # or * P < 0.05, ## or ** P < 0.01, ### or *** P < 0.001, #### or **** P < 0.0001