Fig. 2

HBO1-mediated histone acetylation in DNA replication. HBO1-mediated histone acetylation promotes G1 phase DNA replication licensing and S phase DNA replication activation. In the G1 phase, the HBO1–JADE complex promotes the acetylation of H4K5/8/12 to relax the chromatin conformation, and facilitates the loading of the MCM complex to the replication starting point, promoting the assembly of the pre-replication complex. In S phase, the HBO1–BRPF3 complex specifically directs H3K14ac, thereby promoting CDC45 loading to activate S-phase DNA replication. Additionally, the regulatory protein Geminin prevents the second round of DNA replication by inhibiting the basic replication factor CDT1, possibly by inhibiting HBO1