Fig. 3

MiR-335-3p repressed SERPINE2 expression by targeting the 3′-UTR of SERPINE2. A The bioinformatic analysis showed SERPINE2 was the potential target of miR-335-3p. B–D The mRNA and protein expression of SERPINE2 in LF tissues were evaluated through the qRT-PCR and IHC. E–G The mRNA and protein expression of SERPINE2 after the miR-335-3p overexpression were assessed using the qRT-PCR and western blot. H–J The relationship among miR-335-3p, SERPINE2, and AGO2 was assessed based on RIP analysis. K The potential binding sites between miR-335-3p and SERPINE2 were predicted using the TargetScanHuman 8.0 database. L, M The binding sites between miR-335-3p and SERPINE2 were identified using the dual luciferase assay. Scale bar, 100 μm. ns, not significant, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001