Fig. 6

Analysis of mitochondrial function and protein expression in response to NaOH-Aβ42 and NH4OH-Aβ42 treatment. A MitoTracker analysis showed a decrease in mitochondrial membrane potential in cells exposed to NaOH-Aβ42 after 4 days (85.01%, n = 3, p = 0.02525), although mitochondrial mass had not changed. B The expression levels of F-ATPase showed a significant decrease in NaOH-Aβ42-treated cells after 4 days (71.14%, n = 3, p = 0.00847), while they remained unchanged when NH4OH-Aβ42 was applied. Conversely, mt-TFA levels increased only in NaOH-Aβ42 experiments after 2 days (139.18%, n = 4, p = 0.01517) and 4 days (139.18%, n = 4, p = 0.00054) of amyloid exposure. Statistical significance level versus untreated samples: *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001