Table 3 Information on all plasmids
From: The miR-1269a/PCDHGA9/CXCR4/β-catenin pathway promotes colorectal cancer invasion and metastasis
Plasmid | Company |
|---|---|
PCDHGA9 vector (VPCDHGA9) (pLenti-CMV-MCS-3FLAG-PGK-Puro) | OBiO |
PCDHGA9 OE (OEPCDHGA9) (pLenti-CMV- PCDHGA9-3FLAG-PGK-Puro) | OBiO |
PCDHGA9 NC (Plvx-NC) | OBiO |
PCDHGA9 Sh (Plvx-shRNA1, Target sequence: CCCAAATTCTTGACCGAGAAA) PCDHGA9 Sh2 (Plvx-shRNA2, Target sequence: GCGGAAGATTAGTCCTGCTAT) PCDHGA9 Sh3 (Plvx-shRNA3, target sequence: CCTGCAAGTGACTGACATCAA) | OBiO |
CXCR4 vector (pLenti-CMV-MCS-3FLAG-PGK-Puro) | OBiO |
CXCR4 OE (pLenti-CMV-CXCR4-3FLAG-PGK-Puro) | OBiO |
HOXA1 vector (pLenti-CMV- MCS-6HIS-PGK-Puro) | OBiO |
HOXA1 OE (pLenti-CMV-HXA1-6HIS-PGK-Puro) | OBiO |
PCDHGA9-WT- miR-1269a (GP-miRGLO, ctaacaggac caatggatta aactggcatt tcagtccaag gaagctcgaa gcaggtttag PCDHGA9-MUT- miR-1269a (GP-miRGLO, ctaacaggac caatggatta aactgCGTtt tGTCAGGTag gaagctcgaa gcaggtttag) | Genepharma |
CXCR4 WT (pGL4.10-CXCR4 promoter (WT), chr2:136118150–136120149) | OBiO |
CXCR4 MUT1 (pGL4.10-CXCR4 promoter (MUT1), chr2:136118150–136119049) | OBiO |
CXCR4 MUT2 (pGL4.10-CXCR4 promoter (MUT2), chr2:136118150–136118884) | OBiO |
miR-1269a vector (VmiR-1269a) (pSLenti-EF1- Luc2-P2A-BSR-CMV-MCS-WPRE) | OBiO |
miR-1269a OE (OEmiR-1269a) (pSLenti-EF1- Luc2-P2A-BSR-CMV- miR-1269a-WPRE) | OBiO |