Table 2 Information on the antibodies
From: The miR-1269a/PCDHGA9/CXCR4/β-catenin pathway promotes colorectal cancer invasion and metastasis
Primary antibody | Dilution ratio | Company |
|---|---|---|
Anti-PCDHGA9 | 1:1000 | Abnova |
Anti-GAPDH | 1:1000 | Cell Signaling Technology |
Anti-β-catenin | 1:1000 | Cell Signaling Technology |
Anti-E-cadherin | 1:1000 | Cell Signaling Technology |
Anti-N-cadherin | 1:1000 | Cell Signaling Technology |
Anti-vimentin | 1:1000 | Cell Signaling Technology |
Anti-Snail | 1:1000 | Cell Signaling Technology |
Anti-CXCR4 | 1:500 | Abclonal |
Anti-HOXA1 | 1:500 | Affinity |
Anti-Flag | 1:1000 | Sigma |
Anti-LaminA | 1:1000 | Cell Signaling Technology |
Anti-β-tubulin | 1:1000 | Cell Signaling Technology |
Second antibodies | ||
Goat anti-mouse IgG (H+L) | 1:10,000 | Jackson |
Mouse anti-rabbit IgG (conformation specific) mAb | 1:1000 | Cell Signaling Technology |