Fig. 7

The regulatory relationship among PCDHGA9, miR-1269a, CXCR4, and β-catenin. a Quantitative PCR (qPCR) analysis demonstrated the expression levels of miR-1269a following the downregulation or overexpression of CXCR4. b Western blotting (WB) revealed the protein expression of CXCR4 and β-catenin in the presence or absence of the HOXA1 overexpressing plasmid. c WB showed alterations in the protein levels of HOXA1, CXCR4, and β-catenin upon PCDHGA9 overexpression and the introduction of the miR-1269a mimic. d WB depicted changes in the HOXA1 distribution between the cytoplasm and nucleus in the presence or absence of PCDHGA9 overexpression and the miR-1269a mimic. e WB analysis demonstrated that (E)-ferulic acid, a β-catenin inhibitor, affected β-catenin expression without impacting PCDHGA9 and CXCR4. Similarly, CHIR-99021, a β-catenin activator, influenced β-catenin expression while leaving PCDHGA9 and CXCR4 unaffected. f A schematic diagram illustrating the regulatory network involving miR-1269a, PCDHGA9, HOXA1, CXCR4, and β-catenin