Fig. 5
From: The miR-1269a/PCDHGA9/CXCR4/β-catenin pathway promotes colorectal cancer invasion and metastasis

CXCR4 is downregulated by PCDHGA9 interacting with HOXA1. a Co-IP assays revealed the presence of the FLAG and HOXA1 proteins in both the input and immunoprecipitated samples. b Dual luciferase assays showing the relative activity of the wild-type (WT) CXCR4 luciferase reporter plasmid after either overexpressing or downregulating PCDHGA9. c Predicted binding sites of HOXA1 within the CXCR4 promoter region at positions 1437, 400, and 77. Two mutant constructs, MUT1 and MUT2, were generated for the three predicted binding sites. Dual luciferase assays were conducted to assess the relative activity of WT, MUT1, and MUT2 CXCR4 luciferase reporter plasmids in the presence or absence of HOXA1. d Western blot analysis depicting the protein expression of CXCR4, PCDHGA9, and β-catenin in CRC cells with altered CXCR4 expression