Fig. 4

MiR-1269a promotes invasion, metastasis, and EMT of CRC cells. a Wound healing assays were conducted in CRC cells transfected with either miR-1269a inhibitor or mimic, along with their respective controls. The widths of wounds were measured at 0 h and 48 h, and the percentages of wound closure were calculated and analyzed. b Transwell assays were performed in CRC cells transfected with either miR-1269a inhibitor or mimic, along with their respective controls. The cells that had migrated out of the chamber were observed and counted. c Western blotting results demonstrated changes in the protein levels of E-cadherin, N-cadherin, vimentin, and Snail after transfection with either miR-1269a inhibitor or mimics into cells