Fig. 3

miR-1269a targets PCDHGA9 and downregulates PCDHGA9. a Venn diagram illustrating the microRNAs predicted to interact with PCDHGA9 across three databases (starBase, Targetscan, and miRBD). Among these, hsa-miR-1269a, hsa-miR-5586-5p, hsa-miR-580-3p, hsa-miR-760, hsa-miR-625-5p, and hsa-miR-3179 were identified as commonly predicted microRNAs. b PCDHGA9 expression was assessed via qPCR after transfection of miR-1269a and miR-3179 mimics into 293T cells. c Four groups of 293T cells were transfected with different combinations of vectors (NC or miR-1269a mimic) and luciferase reporter plasmids (wild type or mutant type PCDHGA9). The luciferase activity was measured using the dual luciferase assay system. d The relative expression of PCDHGA9 was examined by qPCR following the transfection of miR-1269a inhibitor or mimic into CRC cells. e The protein levels of PCDHGA9 and β-catenin were analyzed by western blotting in CRC cells transfected with miR-1269a inhibitor or mimic. f The interacting region between PCDHGA9 and miR-1269a is shown