Fig. 2

PCDHGA9 suppresses invasion, metastasis, and EMT of CRC cells. a Wound healing assays were conducted on CRC cells with overexpressed or downregulated PCDHGA9 and their respective controls. The width of wounds was measured at 0 and 48 h, and the percentage of wound closure was calculated and analyzed using the Student’s t-test. b Transwell assays were performed on CRC cells with manipulated PCDHGA9 expression and their controls, and the number of cells that had migrated through the chamber was observed and quantified. c Western blot analysis showing changes in E-cadherin, N-cadherin, vimentin, and Snail expression following the overexpression or downregulation of PCDHGA9. Notably, E-cadherin was not detected in RKO cells from our laboratory, resulting in the absence of an E-cadherin blot