Fig. 1
From: The miR-1269a/PCDHGA9/CXCR4/β-catenin pathway promotes colorectal cancer invasion and metastasis
Comprehensive investigation of PCDHGA9 in CRC: expression in tissue, survival analysis, cell line manipulation, and high-throughput sequencing results. a Real-time quantitative PCR (qPCR) revealed decreased PCDHGA9 expression in 42 CRC tissues among 63 paired samples, compared with adjacent normal tissues. b, c Patients with low PCDHGA9 expression exhibited shorter overall survival and disease-free survival rates. d, f Western blot analysis assessed PCDHGA9 expression in HCT116, RKO, HCT8, and SW620 cells, as well as the impact of overexpressing or knocking down PCDHGA9 on PCDHGA9 and β-catenin expression. e Immunohistochemistry exhibits the expression of PCDHGA9 in CRC tissues with low, moderate, and high differentiation. g High-throughput sequencing identified differential gene expression between vector and PCDHGA9-overexpressing cells, with downregulated genes listed on the right