Fig. 5

Low deformability of LSECs from Mcpip1^fl/fl LysM^Cre mice indicates altered forced sieving. A Representative images of individual sieve plates measured in living LSEC using QI AFM. The images are reconstructed for load force of 170 nN and 300 pN which represents ~ 50% and ~ 80% of the maximum load force, respectively. B Selected cross-section indicated in (A) was presented showing the differences between the load force used. The enlargement of fenestrations was greater in the control rather than the Mcpip1 KO group, which was schematically illustrated in (C). D shows the distribution of > 200 fenestrations compared in a one-to-one manner for selected load force values with a linear fit. E The scheme illustrating the forced sieving of lipoproteins through fenestrations is a modification of a scheme shown in [59]. n = 3 animals