Fig. 2

Comparison of LSEC porosity measurements in Mcpip1 KO and control mice using AFM and SEM. A Representative scanning electron microscopy images of LSECs isolated from 3-month-old and 6-month-old mice. B,C The porosity of LSECs expressed as a fenestrae frequency, i.e. the number of fenestrations per area of cell, as measured using (B) atomic force microscopy (AFM) and (C) scanning electron microscopy (SEM) for GA-fixed cells. Additionally, the effect of 30 min treatment with 21 µM cytochalasin B was presented, showing weaker responsiveness, i.e. induction of less fenestrae in the Mcpip1 KO group. Each point represents a single cell. The box charts show mean ± 25–75% of data points and the whiskers 5% and 95%. * p < 0.05; ** p < 0.01, *** p < 0.001. Additionally, # indicates significance of p < 0.01 after cytochalasin B treatment versus respective non-treated genotype. n = 3–6 animals