Fig. 1
Topography images of representative LSECs isolated from 3-month-old and 6-month-old Mcpip1 KO and control mice were measured using QI AFM. A The first row represents LSEC isolated from control, Mcpipfl/fl mice; the second row from Mcpip1 KO, Mcpip1fl/fl LysMCre mice. Additionally, the effect of 30 min treatment with 21 µM cytochalasin B was presented, showing weaker responsiveness, i.e. induction of fewer fenestrae in the Mcpip1 KO group. Images are in scale for comparison. 30 × 35 µm2, 350 × 408 pixels. B High-magnification images of untreated and cytochalasin B-treated LSECs from the Mcpip1 KO group. Sieve plates without opened fenestrations were indicated using asterisks, small arrows show examples of cytoskeletal fibres that were visible only in the untreated group. A large arrow indicates a sieve plate filled with fenestrations. ‘G’ indicates a gap, a large hole that was not classified as functional fenestration. Images are in scale for comparison. 18 × 16 µm.2, 204 × 184 pixels (bicubic interpolation). C Cell height of control and Mcpip1 KO LSECs in both time points (n = 3 animals). ** p < 0.01