Fig. 7
Correlation between D2-like receptor desensitization and EGF-induced GPCR-EGFR interaction. HEK-293 cells were transfected with EGFR-GFP along with corresponding FLAG-tagged D2-like receptors. Cells were treated with either vehicle or 10 ng/mL of EGF for 5 or 20 min. The cell lysates were immunoprecipitated with FLAG beads. Co-IP/lysate and IP were immunoblotted with antibodies against GFP and FLAG. A FLAG-tagged D2R and D3R were used. *p < 0.05 compared with other groups of the cells expressing D2R or D3R (n = 3). B FLAG-tagged D3R and D4R were used. *p < 0.05, **p < 0.01 compared with other groups of the cells expressing D3R or D4R (n = 3). C FLAG-tagged WT-D3R and C147K-D3R were used. *p < 0.05, **p < 0.01 compared with other groups of the cells expressing WT-D3R or C147K-D3R (n = 4). D FLAG-tagged WT-D2R and K149C-D2R were used. ***p < 0.001 compared with the groups of WT-D2R-expressing cells; ##p < 0.01 compared with other groups of K149C-D2R-expressing cells (n = 4)