Fig. 6

Induction of the GPCR desensitization causes the interaction between D2-like receptors. HEK-293 cells were transfected with EGFR-GFP along with FLAG-tagged D2-like receptors. “ + ” and “w/+” represent the cells treated with the corresponding agonist for 5 min and washed/rechallenged, respectively. The cell lysates were immunoprecipitated with FLAG beads. Co-IP/lysate and IP were immunoblotted with antibodies against GFP and FLAG. A FLAG-tagged D₂R and D₃R were used. Cells were treated with 10 μM of dopamine. **p < 0.01 compared with other groups of the cells expressing D₃R or D₄R (n = 3). B FLAG-tagged D₃R and D₄R were used. Cells were treated with 10 μM of dopamine. **p < 0.01 compared with other groups of the cells expressing D₃R or D₄R (n = 4). C FLAG-tagged WT-D₃R and C147K-D₃R were used. Cells were treated with 100 nM of quinpirole. **p < 0.01 compared with other groups of the cells expressing WT-D₃R or C147K-D₃R (n = 4). D FLAG-tagged WT-D₂R and K149C-D₂R were used. Cells were treated with 10 μM of dopamine. **p < 0.01 compared with other groups of the cells expressing WT-D₂R or K149C-D₂R (n = 3)