Fig. 1
Transactivation of EGFR is involved in D3R desensitization. A HEK-293 cells expressing D3R (1.7–2.3 pmol/mg protein) were pretreated with vehicle or 200 nM AG1478 for 30 min, followed by desensitization induction (w/+). Quin (+) and Quin (w/+) represent the cells treated with 100 nM of quinpirole for 5 min and washed/rechallenged, respectively. **p < 0.01, ***p < 0.001 compared with other groups at 10–10–10−8 M Quin (n = 3). B Cells were transfected with HA-Src. The cell lysates were immunoblotted with antibodies against p-Src (Y416) and HA. **p < 0.01 compared with other groups except Veh/AG1478 group; #p < 0.05 compared with Veh/AG1478 group (n = 4). C HEK-293 cells expressing D3R were transfected with FLAG-PDK1. The cell lysates were immunoprecipitated with FLAG beads. IPs were immunoblotted with antibodies against p-PDK1 (S241) and FLAG. **p < 0.01 compared with Veh/Veh group; #p < 0.05 compared with other groups except Veh/Veh group (n = 4). D HEK-293 cells expressing D3R were transfected with HA-Akt. The cell lysates were immunoblotted with antibodies against p-Akt (T308) and HA. **p < 0.01 compared with other groups (n = 4)