Fig. 5
From: The miR-6240 target gene Igf2bp3 promotes myoblast fusion by enhancing myomaker mRNA stability
Igf2bp3 regulates the expression of Mymk through affecting mRNA stability. A The expression correlation between Igf2bp3 and Mymk in skeletal muscle. B, C The Mymk mRNA (B) and protein (C) expressions were assessed in the KO-Igf2bp3 C2C12 cell line on day 5 of differentiation. D, E The KO Igf2bp3-C2C12 cell line was transfected with pcDNA3.1(+)-Igf2bp3 or a negative control plasmid, followed by assessment of Mymk mRNA (D) and protein (E) levels. F, G Potential m6A modification site (F) and secondary structure (G) of Mymk predicted by the SRAMP website. H The enrichment of Mymk in the RIP experiment. I The stability of the Mymk mRNA was impaired by the knockdown of Igf2bp3. J The stability of the Mymk mRNA was enhanced by the overexpression of Igf2bp3. K The stability of the Mymk mRNA was significantly reduced in the KO-Igf2bp3 C2C12 cell line. L The KO-Igf2bp3-C2C12 cell was transfected with pcDNA3.1(+)-Igf2bp3 or a negative control plasmid, followed by assessment of Mymk mRNA stability. GAPDH was utilized as an internal control. N = 3 in each group. Data are represented as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant (Student’s t-test)