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Fig. 2 | Cellular & Molecular Biology Letters

Fig. 2

From: The miR-6240 target gene Igf2bp3 promotes myoblast fusion by enhancing myomaker mRNA stability

Fig. 2

The function of Igf2bp3 on myoblast fusion. A, B mRNA (A) and protein (B) levels of Igf2bp3 and fusion markers were measured by RT-qPCR and western blot following transfection with Igf2bp3 siRNA or a negative control. C Immunofluorescence detection of MyHC (green) in C2C12 myoblasts transfected with Igf2bp3 siRNA or a negative control. To detect cell nuclei, DAPI (blue) was utilized. Measurements were made of the fusion index and the myotube diameter. D, E The expression levels of Igf2bp3 and fusion markers were measured by RT-qPCR (D) and western blot (E) after Igf2bp3 overexpression. F Immunofluorescence analysis of C2C12 myoblasts overexpressing Igf2bp3, stained with MyHC (green). The cell nuclei were visible with DAPI (blue). For C and F, micrographs of MyHC and merge were taken using 200× magnification (scale bar, 100 µm) and magnified the partial part of the merge image was magnified by 4× (scale bar, 25 µm). Gapdh or α-tubulin was utilized as an internal control. N = 3 in each group. Data are represented as mean ± SEM. **P < 0.01; ***P < 0.001 (Student’s t-test)

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