Fig. 1

The expression and cellular localization of Igf2bp3. A The Igf2bp3 expression pattern in various tissues of 12-week-old mice. B The expression of Igf2bp3 during regeneration is based on the SCSMRD database. C, D RT-qPCR and western blot to quantify the Igf2bp3 mRNA (C) and protein (D) levels in C2C12 cells that were differentiating and proliferating, respectively. E, G The distribution of Igf2bp3 in the nucleus (blue) and cytoplasm (red) was detected by RT-qPCR after purification of nuclear and cytoplasmic RNAs from proliferating (E) and differentiating (G) C2C12 myoblasts. F, H Western blot to quantify IGF2BP3 expression in the nucleus and cytoplasm of proliferating (F) and differentiating (H) C2C12 myoblasts. I IGF2BP3 subcellular location in C2C12 myoblasts was identified using immunofluorescence. DAPI-stained nuclei are shown in blue, while IGF2BP3 staining is shown in red. Scale bars, 100 μm. For normalization, Neat1, GAPDH, and histone H3 were employed. N = 3 in each group. Data are represented as mean ± SEM. ***P < 0.001 (Student’s t-test)