Fig. 2
Akt1s1 deletion impairs renal manifestations of Tsc2-deleted male mice. A. Mating strategy of the mice. B. Genotyping results. C. Images of male mice and their kidneys. D. Ratios of kidney weight to body weight. n = 3 for Akt1s1+/+Tsc2f/+; Cre mice; n = 10 for Akt1s1+/+Tsc2f/f; Cre mice; n = 5 for Akt1s1−/−Tsc2f/+; Cre mice; n = 12 for Akt1s1−/−Tsc2f/f; Cre mice. E. Hematoxylin and eosin (HE) staining of the kidneys. Scale bars, 1000 µm. F. Cystic indexes. n = 3 for Akt1s1+/+Tsc2f/f; Cre mice; n = 4 for Akt1s1−/−Tsc2f/f; Cre mice. G. BUN values. n = 3 for Akt1s1+/+Tsc2f/+; Cre and Akt1s1−/−Tsc2f/+; Cre mice; n = 5 for Akt1s1+/+Tsc2f/f; Cre and Akt1s1−/−Tsc2f/f; Cre mice. H. IHC staining with anti-PCNA antibody. Scale bars, 50 µm. I. Percentages of the PCNA positive cells in the epithelial cells of the cysts. J. Immunoblotting analysis for the kidneys. K. IHC staining with the indicated antibodies. Scale bars, 50 µm. The quantification values of the band density were labeled above the bands. Bars, SD. *P < 0.05, **P < 0.01, ***P < 0.001 from Student’s t-test or one-way ANOVA