Fig. 1

Association of PRAS40 expression with mTOR activation. Immunoblotting (A–C, E, F, H–K) or real-time PCR analysis (D, G). Tissue lysates were extracted from kidneys of Tsc2^f/+; ksp-cre and Tsc2^f/f; ksp-cre mice (A) or kidneys of Tsc2^f/f; ksp-cre mice treated with or without rapamycin (B). Tsc2^+/+ or Tsc2^−/− MEFs were cultured in the media with (+) or without serum (−) (C, D). Pten^−/− MEFs were cultured in the media with (+) or without serum (−) (E). Tsc2^−/− MEFs (F, G), HK2 (H), NRK (I), and 786–0 (J) cells were treated with or without rapamycin (5 nM) for 24 h. Tsc2^−/− MEFs were transfected with control shRNA, mTOR shRNA1, or mTOR shRNA2 (K). The quantification values of the band density were labeled above the bands. Bars, SD. ***, P < 0.001 from Student’s t-test