Fig. 7

Nrf1 acetylation affects Nrf1 stability and microglial activation. A Lysine mutation design of Nrf1. B Luciferase activity was measured in microglia transfected with a murine p65 promoter–luciferase reporter constructed along with WT Nrf1 or Nrf1 mutants. HDAC3 was co-expressed as indicated. The p65 promoter reporter activity was normalized to Renilla luciferase activity and empty vector (NC) activity. Luciferase activity was measured and analysed. An empty vector was used as a control, and pairwise comparisons were made between the glutamate and arginine mutants for each amino acid site (n = 5 per group). C Protein stability in microglia transfected with the K105Q, K105R, K139Q and K139R mutants after treatment with 100 μg/ml CHX. Samples were obtained 0 and 90 min after CHX treatment (n = 3 per group). In microglia with OGD/R-induced injury, the influence of the Nrf1 K105R and/or K139R mutations on the improvement observed after PTS treatment were evaluated by WB analysis of iNOS and Arg1 (D) and ELISA analysis of iNOS activation (E). The data are presented as the means ± SEMs (n = 3). *p < 0.05, versus the control group; #p < 0.05, versus the OGD/R + vehicle group; p < 0.05, versus the OGD/R + PTS group