Fig. 6

PTS improves Nrf1 acetylation and microglial activation by inhibiting HDAC3. A Nrf1 acetylation in the cell nucleus of ischaemic brain tissue was detected by a co-IP assay 24 h after MCAO/R. The data are presented as the means ± SEMs (n = 5). *p < 0.05, versus the sham group; #p < 0.05, versus the MCAO/R + vehicle group; p < 0.05, versus the MCAO/R + ITSA1 group. After OGD/R, microglia were immediately treated with PTS, ITSA1 + PTS, or vehicle for 24 h in vitro. Then, the acetylation of Nrf1 in the cell nuclei of microglia was detected by co-IP (B). Total HDAC activation (C), iNOS activation (D) and the levels of the inflammatory factors TNF-α and IL-1β were determined by ELISA (E), and WB analysis of iNOS and Arg1 expression (F) was performed using total cell lysates from OGD/R-induced microglia. The data are presented as the means ± SEMs (n = 3). *p < 0.05, versus the control group; #p < 0.05, versus the OGD/R + vehicle group; p < 0.05, versus the OGD/R + PTS group