Fig. 2

HDAC3 regulates Nrf1 expression and iNOS activation after I/R. After MCAO/R mice were treated with the HDAC activator ITSA1 and the HDAC3 inhibitor RGFP966, we conducted qRT‒PCR analysis of HDAC1, HDAC2, HDAC3 and Nrf1 mRNA levels (A); WB analysis of HDAC1, HDAC2 and HDAC3 expression (B); WB analysis of Nrf1, iNOS and Arg1 expression (C); and ELISA analysis of iNOS activation (D) in total cell lysates of ischaemic brain tissue 24 h after MCAO/R. The data are presented as the means ± SEMs (n = 5). *p < 0.05, versus the sham group; #p < 0.05, versus the MCAO + vehicle group; &p < 0.05, versus the MCAO/R + PTS group