Fig. 5
From: CLDN6 inhibits breast cancer growth and metastasis through SREBP1-mediated RAS palmitoylation
CLDN6 restrains SREBF1 transcription via modulating KLF5 nuclear translocation. A The SREBF1 expression in the Timer database was positively associated with the KLF5 expression in stromal BC. B, C The most likely binding site of KLF5 to the SREBF1 promoter predicted by the JASPAR database. D ChIP was used to detect the binding of KLF5 to SREBF1 promoter in BC cells. E, F Dual-luciferase reporter assay of BC cells transfected with WT or Mut SREBF1 promoter reporter plasmid and KLF5 plasmid (n = 3). G The impact of CLDN6 overexpression on KLF5 (red) positioning in BC cells. Scale bar, 20 μm. H The modulation of CLDN6 on the KLF5 nuclear and cytoplasmic distribution (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 denoted the presence of statistically significant disparities