Fig. 9

Inhibition of hnRNPA1 reversed the effects induced by SRPK1-expression. A hnRNPA1 knockdown suppress SRPK1-induced cell proliferation, as detected by CCK-8 assays. B, D hnRNPA1 knockdown suppress SRPK1-induced cell proliferation, as detected by EdU assays. C hnRNPA1 knockdown suppress SRPK1-induced high lactate production and glucose uptake. E hnRNPA1 knockdown suppress SRPK1-induced overexpression of hnRNPA1 and PKM2, and downregulation of PKM1. Total PKM level showed no significant difference. F Knockdown of SRPK1 and hnRNPA1 inhibits METTL3-induced overexpression of PKM2 and downregulation of PKM1. Total PKM level showed no significant difference. Data represent the mean (± SD) of three independent experiments, and two-way ANOVA and unpaired t-tests were carried out to validate the statistical significance. *P < 0.05; **P < 0.01; ***P < 0.001