Fig. 5
From: Biophysical characterization of the phase separation of TDP-43 devoid of the C-terminal domain
Electrostatic forces governing phase separation of PrLD-less, PrLD and full-length TDP-43. A Domains composing full-length (centre), PrLD-less (left) and PrLD (right) TDP-43. Net charges of individual domains at neutral pH are negative if featuring low pI (red), weakly positive with moderately high pI (pale blue) and positive with very high pI (blue). pIs are indicated (pIs with a 6 His-tag in brackets). Numbers below domains indicate residue boundaries. B Types of phase separated assemblies observed early (top) and later (bottom) for the TDP-43 constructs. Only PrLD-less (left) and PrLD (right) TDP-43 form genuine liquid droplets. Full-length TDP-43 forms gel-like droplets with limited recovery of RFI after photobleaching that cluster further without fusion (middle). C Factors governing phase separation for the three constructs. D Schematics of electrostatic forces governing phase separation for the three constructs (colours as in A)